Fig. 8: Desmin affects active and inactive chromatin compartments of cardiomyocytes at a megabase scale. | Cell Death & Disease

Fig. 8: Desmin affects active and inactive chromatin compartments of cardiomyocytes at a megabase scale.

From: Molecular insights into the role of desmin intermediate filament network in chromatin landscape, cardiomyocyte differentiation, and maturation

Fig. 8

A A/B compartment heatmaps after FAN-C analysis of cardiomyocyte genomes from Des+/+ and Des−/− backgrounds at different time points. (Ai) on iCMs: early in trans-differentiation at D7 (condition ‘a’), (Aii) on NCM, at day 1-3 post birth (neonatal) (condition ‘b’), (Aiii) on ACM isolated from 3month old mice (adulthood) (condition ‘c’) and (Aiv) on ACM swim: isolated from 3month old mice undergone a swimming exercise stress (condition ‘d’). Resolution 1 mb. B Representative of FAN-C analysis at 10Kb resolution of the genome contact matrix at the conditions mentioned in (A) on specific chromosomes where genes of interest are located. Shown: α-actinin and desmoplakin (chr13:38,151,294-38,198,577 and chr13:12,269,426-12,340,760), Connexin 43 or Gja1 (chr10:56,388,423-56,388,482), Notch1 (chr2:26,459,531-26,503,558). Long light blue boxes cover the locations of the specific genes. The color maps represent relative chromatin interaction probability and are displayed on the same scale for each condition for comparison. iCMs induced cardiomyocytes, NCMs neonatal cardiomyocytes, ACMs adult cardiomyocytes, Genome browser (https://genome.ucsc.edu) was used for gene locations.

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