Fig. 4: Mild ER stress is needed for an effective IL-1βlow-induced hormesis in INS-1E cells. | Cell Death & Disease

Fig. 4: Mild ER stress is needed for an effective IL-1βlow-induced hormesis in INS-1E cells.

From: IL-1β priming triggers an adaptive stress response that enhances pancreatic β-cell resilience to subsequent cytotoxic inflammatory insult

Fig. 4

INS-1E cells were conditioned with IL-1βlow in the absence or presence of tauroursodeoxycholic acid (TUDCA, 5 μM) and subsequently challenged or not with CYT for 16 h. A NO levels in the culture media were quantified by the Griess reaction and normalized to total cell protein content, n = 3. B–E Protein levels of ATF4, CHOP, and cleaved caspase-3 were analyzed by Western blot. β-actin was used as a loading control, n = 5–6. Data are shown as mean ± SD. (*) p < 0.05, (**) p < 0.01, (***) p < 0.001. (ns) not significant.

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