Fig. 7: IL-1βlow’s effects on gene transcripts associated with β-cell identity and function in murine islets. | Cell Death & Disease

Fig. 7: IL-1βlow’s effects on gene transcripts associated with β-cell identity and function in murine islets.

From: IL-1β priming triggers an adaptive stress response that enhances pancreatic β-cell resilience to subsequent cytotoxic inflammatory insult

Fig. 7

Murine islets (50 IEQ/well) were preconditioned with IL-1βlow and subsequently challenged or not with IL-1β 100 pg/ml + IFN-γ 5 ng/ml + TNF-α 8 ng/mL (iCYT) for 16 h. An additional condition involving a 24 h washout period (without iCYT) was also evaluated. A Ucn3 mRNA, B Pdx-1 mRNA, C Glut2 mRNA, D Ins1 mRNA, E Ins2 mRNA, F BIP mRNA and G Aldh1a3 mRNA expression were analyzed by RT-qPCR. Relative mRNA levels were normalized to HPRT, n = 3–8. Data are shown as mean ± SD (*) p < 0.05, (**) p < 0.01, (***) p < 0.001, (ns) not significant.

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