Fig. 2: Intracellular localization of transgenically over-expressed CLN7. | Cell Death & Disease

Fig. 2: Intracellular localization of transgenically over-expressed CLN7.

From: CLN7 protein functions at the interface between endolysosomes and stress granules to promote cell survival

Fig. 2

A iNPCWT, iNOCBD1 and iNPCBD2 were transduced with a lentivirus expressing an mScarlet-CLN7 fusion at 5 MOI and then co-stained with LysoTracker after 4-days following Bafilomycin A (10 nM) or DMSO carrier treatment for 12 h. The panel shows phase contrast, mScarlet-CLN7 (red) and LysoTracker (green) imaging of living cells. Size bar=30 µm. Corrected total cell fluorescence (CTCF) was quantified and compared for mScarlet-CLN7 (B) and LysoTracker (C) from a minimum of 10 captured images of 3 independent experiments. All data presented as mean ± SEM, *p ≤ 0.05, **≤0.01, ***≤0.001 and ****≤0.0001, (p-values in Table 8) Size bar = 30 µm.

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