Fig. 6: Unbiased global proteomics comparison of iNPC^BD to iNPC^WT with and without BafA treatment.

A Schematic workflow of iTRAQ mass spectrometry, proteomic quantification and downstream bioinformatic analyses. Proteins with differential regulation of >2-fold with a p < 0.05 in iNPC^BD versus iNPC^WT were subjected to hierarchical Bayesian modeling then pathway changes identified using Cytoscape gProfiler through Reactome Pathway. Bi Upregulated protein pathways ranked by p-value and Bii separated by cellular localization. Biii Upregulated protein pathways ranked by p-value following Baf A (10 nM for 12 h) treatment. C A Minimal Essential Network identified in differentially upregulated proteins focussed around vesicular transport. Di Downregulated protein pathways ranked by p-value and Dii separated by cellular localization. Diii Downregulated protein pathways ranked by p-value following Baf A (10 nM for 12 h) treatment. E A Minimal Essential Network identified from differentially downregulated proteins focussed on RNA interactions.