Fig. 1: YTHDC2 is downregulated in BLCA and correlates with better survival.
From: YTHDC2 suppresses bladder cancer by inhibiting SOX2-mediated tumor plasticity
A The mRNA expression level of YTHDC2 in normal and tumor tissues. The plot was generated from TNMplot [60] (https://tnmplot.com/analysis/). Significant differences by Mann–Whitney U test were marked with red*. B The mRNA expression level of YTHDC2 in normal (n = 40) and BLCA (n = 406) tissues from GTEx and TCGA-BLCA datasets. C The mRNA expression level of YTHDC2 in normal (n = 68) and BLCA (n = 188) tissues from GSE13507 dataset. D Expression of YTHDC2 mRNA in NMIBC (n = 103) and MIBC (n = 62) from GSE13507 dataset. E Expression of YTHDC2 mRNA in low grade (n = 105) and high grade (n = 60) BLCA from GSE13507 dataset. F Expression of YTHDC2 mRNA in nonrecurrence (n = 54) and recurrence (n = 39) BLCA from GSE31684 dataset. G Expression of YTHDC2 mRNA in pTa-pT2 (n = 32) and pT3-pT4 (n = 61) BLCA from GSE31684 dataset. H Overall survival analysis in BLCA patients grouped by YTHDC2 expression in TCGA-BLCA dataset. I Differences of disease-specific survival in BLCA patients grouped by YTHDC2 expression (high vs. low: n = 131vs n = 34) in GSE13507 dataset. J Recurrence-free survival in BLCA patients based on YTHDC2 expression in GSE31684 dataset. K Kaplan–Meier analysis (KM analysis) was utilized to compare the difference of overall survival rate between two groups of BLCA patients in stage 4. Patients were stratified by expression level of YTHDC2. HR value and Log-rank P value were shown on the graph. Gene expression and overall survival data came from the public database TCGA-BLCA. L Overall survival analysis in pan-cancer based on YTHDC2 expression in TCGA Pan-Cancer dataset. M Representative IHC staining of YTHDC2 expression in adjacent (n = 13) and tumor (n = 60) tissue from tissue microarrays of bladder cancer patients. Scatter dot plot analysis of YTHDC2 expression from the tissue microarrays are shown on the right. Scale bar: 50 μm. N Representative images of YTHDC2 IHC staining in tissues from different bladder cancer subtypes (left), and the quantification of the staining by IHC score. Scale bar: 50 μm. Data were analyzed with Mann–Whitney U test. NIPUC non-invasive papillary urothelial carcinoma, IUC invasive urothelial carcinoma. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
