Fig. 3: DDX3X promotes HCC proliferation, migration and invasion in vitro.
From: Targeting FAM134B-DDX3X axis inhibiting AKT signaling in hepatocellular carcinoma
A Western blot detected whether DDX3X overexpression and knockdown cell lines were successfully constructed, and detected the phosphorylated AKT (Ser473) level. B−F CCK-8 assay, n = 5 (B), soft agar assay, n = 3 (C, D) colony formation assay, n = 3 (E, F) detected the indicated cell line proliferation rate. Data are mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001. G Flow cytometry tested the effect of DDX3X reduction on the cell cycle of MHCC97-H cells. Data are mean ± SEM from three independent experiments, **P < 0.01. H Western blot detected the expression of cell cycle related proteins in the indicated cells. I−K Transwell assay detected the effect of DDX3X on HCC cells migration and invasion. Data are mean ± SEM from at least three independent experiments, ***P < 0.001.
