Fig. 5: FAM134B promotes HCC progression via DDX3X.
From: Targeting FAM134B-DDX3X axis inhibiting AKT signaling in hepatocellular carcinoma
A Western blot detected the protein level of FAM134B, DDX3X, p-AKT (Ser473), AKT in rescue cell lines. B−F CCK-8 assay, n = 5 (B), colony formation assay, n = 3 (C and D) investigated whether the promotional effect of FAM134B on HCC proliferation was dependent on DDX3X. Data are mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001. E−H Transwell assay investigated whether the promotional effect of FAM134B on HCC migration and invasion was dependent on DDX3X. Data are mean ± SEM from three independent experiments, *P < 0.05, **P < 0.01, ***P < 0.001. I−M The gross image (I), tumor volume (J), tumor weight (K), Ki-67 index (L), IHC image (M) of subcutaneous tumor model from shNC, shFAM134B-2 and shFAM134B-2 + DDX3X group, n = 5. Scale bar 100 μm, *P < 0.05, **P < 0.01, *** P < 0.001. N-O H&E image of lungs (N), metastatic modules of lungs (O) of teil vein injection lung metastasis model from shNC, shFAM134B-2 and shFAM134B-2 + DDX3X group. n = 5. Scale bar 2000 μm. Data are mean ± SEM, *P < 0.05, **P < 0.01.
