Fig. 7: DDX3X is upregulated in HCC and associated with FAM134B expression.
From: Targeting FAM134B-DDX3X axis inhibiting AKT signaling in hepatocellular carcinoma
A Western blot detected the protein expression of DDX3X in 20 paired HCC tissues and adjacent non-tumor tissues, arrows indicated non-specific bands. The numbers indicated the relative amount of DDX3X signal compared to the first lane on the left, normalized to β-actin. B The log2 transformed fold change of DDX3X expression in tumor tissues and non-tumor tissues from (A). C The representative images of DDX3X expression in tissue microarray. Scale bar 4000 μm (top), 100 μm (bottom). D DDX3X scoring of paired HCC tissues and adjacent non-tumor tissues. Scale bar represents SEM, ***P < 0.001, n = 122. E Kaplan-Meier curves of the overall survival (OS) rates between groups with differential DDX3X expression. P < 0.0001, n = 112. F Pearson’s correlation analysis of FAM134B and DDX3X protein levels. P = 0.0251. G MHCC97-H and Huh7 cells were transfected with HA-FAM134B. Forty-eight hours later, cells were fixed and incubated with anti-HA and anti-DDX3X antibodies. The expression of HA-FAM134B and DDX3X were observed with fluorescence microscope.
