Fig. 8: PKM2 inhibitor affected the regulatory effect of USP7 knockdown on macrophage polarization in SAP pancreatic tissues. | Cell Death & Disease

Fig. 8: PKM2 inhibitor affected the regulatory effect of USP7 knockdown on macrophage polarization in SAP pancreatic tissues.

From: Ubiquitin-specific protease 7 regulates macrophage polarization via pyruvate kinase M2-mediated metabolic reprogramming in severe acute pancreatitis

Fig. 8

A, B Pancreatic macrophages were isolated and the proportion of CD86/F4/80 and CD206/F4/80 positive cells was measured by flow cytometry, respectively. C Analysis of the number of M1 macrophages in pancreatic tissues based on immunofluorescence detection of CD68/iNOS. D Analysis of the number of M2 macrophages in pancreatic tissues based on immunofluorescence detection of CD68/Arg-1. E iNOS, CD86, Arg-1 and Fizz1 mRNA expression in pancreatic tissues from the results of qPCR. F iNOS, CD86, Arg-1 and Fizz1 protein levels in pancreatic tissues from the results of Western blot. n = 3 for (F), n = 6 for (A–E). *p < 0.05, **p < 0.01, ***p < 0.001.

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