Fig. 1: Regulatory network of 16HBE METex14Del in response to HGF.

A Focus on the largest cluster of the co-regulatory network, showing the influence of TFs on METex14Del cells in the presence and absence of HGF. Circles represent DIRs and the radius of the circle is proportional to the number of target genes regulated by the DIR. Co-regulatory interactions between DIRs are indicated: protein-protein interactions with published evidence (blue lines), transcriptional regulation interactions with published evidence (red arrows), and interactions defined only by the h-LICORN algorithm (gray lines). B DIRS were visualized in a volcano plot from interference network analysis: average difference in influence vs. log10 transformed adj. P-value (regulators with an adj. p-value < 0.05 and an influence difference greater/smaller than 0.5 were considered up-/down-influenced (red/green). C DIRS were visualized in a volcano plot from transcriptomic analysis: log₂ fold change vs. p-value of differential mRNA-level expression (genes with an absolute adj. p-value < 0.05 and a fold change greater/smaller than 1.5 were considered upregulated/downregulated (red/green)). D mRNA-level expression of the six main influent DIRs present in the putative regulatory node, in the presence and absence of HGF (triplicates of n = 3 independent experiments). Significance was determined by unpaired one-tailed t-test with Welch’s correction and data are expressed as mean ± SD.