Fig. 3: Effects of ETS1, FOSL1 and SMAD3 silencing on the expression of their predicted target genes on 16HBE METex14Del cell line. | Cell Death & Disease

Fig. 3: Effects of ETS1, FOSL1 and SMAD3 silencing on the expression of their predicted target genes on 16HBE METex14Del cell line.

From: Comprehensive map of the regulatory network triggered by MET exon 14 skipping reveals important involvement of the RAS-ERK signaling pathway

Fig. 3

A The mRNA-level expression of selected target genes up- or down-regulated in response to HGF was determined by RT-qPCR (triplicates of n = 3 independent experiments). Significance was determined by unpaired one-tailed t-test with Welch’s correction and data are expressed as mean ± S.D. B, C The silencing efficacy of each specific siRNA (siETS1, siFOSL1, siSMAD3), used individually or in combination (siPOOL) was assessed by B RT-qPCR for mRNA-level expression (triplicates of n = 3 independent experiments) and C by Western blotting for protein-level expression (representative results). D mRNA-level expression of up- and down-regulated target genes induced by knockdown of ETS1, FOSL1 and SMAD3 expression (triplicates of n = 2, 3 or 4 independent experiments). Significance was determined by one-way ANOVA test and data are expressed as mean ± SD in panels B and D.

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