Fig. 3: GIPC2 interacts with PKM2 via its PDZ domain. | Cell Death & Disease

Fig. 3: GIPC2 interacts with PKM2 via its PDZ domain.

From: GIPC2 regulation of the PKM2/SREBP1 signaling axis controls adipogenic differentiation of mesenchymal stem cells

Fig. 3

a Schematic diagram of Co-immunoprecipitation (Co-IP) assay followed by mass analysis. b Mass spectrometry (MS) analysis identified PKM2 as a potential interacting partner of GIPC2. c Interaction between GIPC2 and PKM2 assessed using Co-IP assays. d Colocalization of GIPC2 and PKM2 in MSCs revealed through immunofluorescence staining. e Protein structure prediction and molecular docking analysis performed to evaluate the binding affinity between PKM2 and GIPC2. f–h Interaction between f PKM2 and GIPC2 (deletion mutant of GIPC2△PDZ), g PKM2 and GIPC2 (deletion mutant of GIPC2△GH1), and h PKM2 and GIPC2 (deletion mutant of GIPC2△GH2) assessed using Co-IP assays. Error bars: mean ± s.e.m.; Scale bar, 25 μm (d).

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