Fig. 6: GIPC2 promotes adipogenic differentiation in MSCs via the PKM2-SREBP1 axis. | Cell Death & Disease

Fig. 6: GIPC2 promotes adipogenic differentiation in MSCs via the PKM2-SREBP1 axis.

From: GIPC2 regulation of the PKM2/SREBP1 signaling axis controls adipogenic differentiation of mesenchymal stem cells

Fig. 6

a WB analysis of the adipogenic markers PPAR-γ, C/EBP-α, and FABP4 in GIPC2-overexpressing MSCs treated with Fatostatin. b ORO staining of Fatostatin-treated GIPC2-overexpressing UC-MSCs to assess lipid droplet formation after 15 days of adipogenic induction. c WB analysis of nuclear proteins isolated from UC-MSCs undergoing adipogenic differentiation for 15 days was performed to assess the nuclear expression of SREBP1 and PKM2 during the differentiation process. d Mechanism schematic diagram of GIPC2 promoting adipogenic differentiation in MSCs via the PKM2-SREBP1 axis. Statistical analysis: one-way ANOVA (c), two-sided t-test (a, b). Error bars: mean ± s.e.m.; Scale bar, 50 μm (b). a, b “GIPC2” indicates UC-MSCs overexpressing GIPC2, and “Fatostatin” indicates GIPC2-overexpressing MSCs with Fatostatin.

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