Fig. 5: Investigating the regulatory mechanisms of PFKFB3 in CRPC cells.
From: PFKFB3 as a multifaceted driver and therapeutic target in castration-resistant prostate cancer
A Schematic diagram of the shPFKFB3 experiment in DU145 cells for transcriptomic analysis. B Heatmap of differentially expressed genes in DU145 cells following shPFKFB3, based on transcriptomic analysis. C List of differentially expressed genes identified in the transcriptomic analysis. D KEGG pathway enrichment analysis of the differentially expressed genes from the transcriptomic data. E, F Western blot validation of changes in protein expression levels corresponding to the transcriptomic findings. G Densitometric quantification of protein expression changes shown in (E, F). H Molecular docking experiments to identify direct interaction targets of PFKFB3. I Immunofluorescence co-localization of PFKFB3 and PDK1 in DU145 cells following shPFKFB3. J Quantitative analysis of PFKFB3 and PDK1 co-localization in DU145 cells shown in (I). K Co-IP analysis of the interaction between PFKFB3 and PDK1 in DU145 cells following shPFKFB3. L Schematic representation of the molecular mechanism of PFKFB3 in CRPC cells. The data were presented as the mean ± SD values. *P < 0.05; **P < 0.01, ***P < 0.001, ****P < 0.0001, ns no significant difference by One-way ANOVA. Related to Figs. S6 and S7.
