Fig. 5: RIT1 plays a crucial role in the cellular processes regulated by REGγ in chordoma progression.

Western blot analysis of REGγ, RIT1, and β-Actin expression in siNC, siREGγ-1#, and siREGγ-1#+siRIT1-1# U-CH1 cells (A), with statistical results in (B). C A CCK8 assay was used to evaluate the effect of REGγ on cell proliferation in siNC, siREGγ-1#, and siREGγ-1#+siRIT1-1# U-CH1 cells. Western blot analysis of REGγ, RIT1 and β-Actin expression in siNC, siREGγ-1#, and siREGγ-1#+siRIT1-1# MUG-Chor1 cells (D), with statistical analysis results in (E). F A CCK8 assay was used to evaluate the effect of REGγ on cell proliferation in siNC, siREGγ-1#, and siREGγ-1#+siRIT1-1# MUG-Chor1 cells. Colony formation ability in siNC, siREGγ-1#, and siREGγ-1#+siRIT1-1# U-CH1 cells (G), with statistical results in (H). Colony formation ability in siNC, siREGγ-1#, and siREGγ-1#+siRIT1-1# MUG-Chor1 cells (I), with statistical results in (J). Cell apoptosis was assessed by flow cytometry after Annexin V-APC staining in siNC, siREGγ-1#, and siREGγ-1#+siRIT1-1# U-CH1 cells (K), with the ratio of cleaved PARP to full-length PARP (L). Cell apoptosis was evaluated by flow cytometry after Annexin V-APC staining in siNC, siREGγ-1#, and siREGγ-1#+siRIT1-1# MUG-Chor1 cells (M), with the ratio of cleaved PARP to full-length PARP (N). Transwell assays were used to assess migration and invasion in siNC, siREGγ-1#, and siREGγ-1#+siRIT1-1# U-CH1 cells (O), with statistical results in (P). Transwell assays were used to assess migration and invasion in siNC, siREGγ-1#, and siREGγ-1#+siRIT1-1# MUG-Chor1 cells (Q), with statistical results in (R). FL PARP: full-length PARP C PARP: cleaved PARP.