Fig. 6: CELSR2 KD U87 MG cells show slow growth in forming glioma in vivo.

A Schematic diagram of U87 MG cell injection in nude mice. B–E Nude mice were inoculated with U87 MG cells transfected with or without CELSR2-shRNA (CELSR2-KD or control group). After one month of inoculation, tumor nodules appeared smaller (B) and their average weight was significantly lower (C) in the CELSR2-KD group compared to the control group. Dynamical measurement showed a significant decrease in tumor volume in the CELSR2-KD group compared to the control group (D), while animal body weight was comparable between the two groups (E). F, G Immunohistochemical staining of tumor tissues confirmed the downregulation of CELSR2 in the CELSR2-KD group. H In tumor nodules, there were a significant upregulation of p-β-catenin and GSK-3β and a significant downregulation of CELSR2, β-catenin, p-GSK-3β and cyclinD1 in the CELSR2-KD group compared to the control group identified by RT-qPCR. I, J EDU labeling revealed a significant decrease in proliferating tumor cells in the CELSR2-KD group compared to the control group. Scale bar is 50 μm in (F, I). Data are represented as mean ± SEM. Two-way repeated measures ANOVA with Bonferroni’s post hoc correction in (D, E) and Student’s t-test in others, *P < 0.05, **P < 0.01, ***P < 0.001, n = 5 in (B–G), (I) and (J), n = 3 in (H). A created with BioRender.