Fig. 5: Induction of apoptosis by FK002-conjugated EMP2 protein in vitro. | Cell Death & Disease

Fig. 5: Induction of apoptosis by FK002-conjugated EMP2 protein in vitro.

From: Development of antibody drug conjugates targeting epithelial membrane protein 2-highly expressed lung cancer

Fig. 5: Induction of apoptosis by FK002-conjugated EMP2 protein in vitro.

A Cell viability assay of lung cancer cells with high EMP2 expression exposed to FK002-exatecan (0, 0.1, 1, 10, 100, 1000 nM) for 48 h. B Cytotoxicity assay of lung cancer cells with high EMP2 expression exposed to FK002-exatecan (0, 0.1, 1, 10, 100, 1000 nM) for 48 h. C and D Cell cycle kinetic analysis of NCI-H226 cells treated with FK002-exatecan (0, 10, 100 nM) for 12 h. Quantitative data are shown in (D). E and F Flow cytometry of SK-MES-1 cells treated with FK002-exatecan (0, 10, 100 nM) for 48 h. Quantitative data are shown in (F). Data are expressed as mean ± SD of n = 3 independent experiments (A–F). ns not significant; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

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