Fig. 6: KDM6A-pSer829 promotes glycolysis by suppressing PER2 mRNA expression.
From: KDM6A phosphorylation suppresses PER2 to confer a glycolytic vulnerability in HNSCC
A Relative expression of PER2, GLUT1, ALDOA, ENO1, PKM2, LDHA, and MCTs1 in HN6 cells expressing KDM6AS829A, KDM6AS829D, KDM6AS829A + PER2 or KDM6AS829D + PER2 for 24 h (n = 3 per group). B Relative expression of PER2, GLUT1, ALDOA, ENO1, PKM2, LDHA, and MCTs1 in HN6 cells expressing KDM6AS829A, KDM6AS829D, KDM6AS829A + si-PER2 or KDM6AS829D + si-PER2 for 24 h (n = 3 per group). C Upper: The enzyme activity of LDH and PK in HN6 cells expressing KDM6AS829A, KDM6AS829D, KDM6AS829A + PER2, or KDM6AS829D + PER2 for 48 h (n = 6 per group). Lower: Extracellular lactate and intracellular lactate in HN6 cells expressing KDM6AS829A, KDM6AS829D, KDM6AS829A + PER2, or KDM6AS829D + PER2 for 48 h (n = 6 per group). D, E Volume and weight of HN6 xenografts (n = 8 mice per group). F Cartoon illustrating the regulation of glycolysis by KDM6A: after phosphorylation by CDK1, KDM6A binds to SFN and is exported out of the nucleus to inhibit PER2 expression through H3K27Me3 mediation and promote glycolysis. Data represent mean ± SEM. Statistics used unpaired two-tailed t test (A–E). Significance is noted as *p < 0.05, **p < 0.01, ***p < 0.001.
