Fig. 1: sc-tPA is cleaved into tc-tPA by primary cortical neurons via a mechanism acquired during cell maturation. | Cell Death & Disease

Fig. 1: sc-tPA is cleaved into tc-tPA by primary cortical neurons via a mechanism acquired during cell maturation.

From: Neuronal conversion of single-chain tissue-type plasminogen activator into its two-chain form: implications in neurodevelopment, learning, and memory

Fig. 1

A Densitometric quantification of the ratio sc-tPA488/tc-tPA488 (1 µM) normalized to stain free in % of control condition in supernatant of living mature (12 DIV) cortical neurons and representative electrophoresis. B Densitometric quantification of BSA555 (1 µM) normalized to stain free in % of control condition in supernatant of living mature cortical neurons representative electrophoresis. C Densitometric quantification of the ratio sc-tPA488/tc-tPA488 (1 µM) normalized to stain free in % of control condition in supernatant of lysed by freezing mature cortical neurons representative electrophoresis. D Densitometric quantification of BSA555 (1 µM) normalized to stain free in % of control condition in supernatant of lysed by freezing mature cortical neurons representative electrophoresis. E Densitometric quantification of the ratio sc-tPA488/tc-tPA488 (1 µM) normalized to stain free in % of control condition in supernatant of living immature (7 DIV) cortical neurons and representative electrophoresis. F Kinetic representation of the ratio sc-tPA488/tc-tPA488 in mature (12 DIV) and immature (7 DIV) cortical neurons. Data are represented as mean ± SD; n = 8 (A, B); n = 6 (C, D); * p < 0.05; ** p < 0.01; **** p < 0.0001; # p < 0.05 compared to control; ## p < 0.01 compared to control; #### p < 0.0001 compared to control; One-sample Wilcoxon test, Mann-Whitney test, F test.

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