Fig. 4: tPA cleavage requires tPA Kringle 2 domain and is independent from plasminogen LBS. | Cell Death & Disease

Fig. 4: tPA cleavage requires tPA Kringle 2 domain and is independent from plasminogen LBS.

From: Neuronal conversion of single-chain tissue-type plasminogen activator into its two-chain form: implications in neurodevelopment, learning, and memory

Fig. 4

A Densitometric quantification of the ratio sc-tPA488/tc-tPA488 (1 µM) in the presence or not of EACA (2.5 and 25 mM) normalized to stain free in supernatant of living mature cortical neurons and representative electrophoresis. B Densitometric quantification of the ratio sc-tPA488/tc-tPA488 (1 µM) in the presence or not of TXA (2.5 and 25 mM) normalized to stain free in supernatant of living mature cortical neurons and representative electrophoresis. C Densitometric quantification of the ratio sc-tPA488/tc-tPA488 (1 µM) and the Kringle 2-deleted sc-ΔK2-tPA488/ΔK2-tc-tPA488 normalized to stain free in supernatant of living mature cortical neurons and representative electrophoresis. D Densitometric quantification of the ratio sc-tPA488/tc-tPA488 (1 µM) in the presence or not of CpB (10 U/mL) normalized to stain free in supernatant of living mature cortical neurons and representative electrophoresis. E Kinetic representation of the ratio sc-tPA488/tc-tPA488 in mature neurons in the presence or not of EACA. F Kinetic representation of the ratio sc-tPA488/tc-tPA488 in mature neurons in the presence or not of TXA. G Kinetic representation of the ratio sc-tPA488/tc-tPA488 and sc-tPA-ΔK2488/tc-tPA-ΔK2488 in mature neurons. H Kinetic representation of the ratio sc-tPA488/tc-tPA488 in mature neurons, in the presence or not of CpB. Data are represented as mean ± SD; n = 5 (A, B, D–F, H); n = 4 (A, B, C, E–G); * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; One-sample Wilcoxon test, Mann-Whitney test, F test.

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