Fig. 4: Integrated multi-omics analysis of SETD7-mediated H3K4me1 modification and transcriptional profiles in ESCC cells.

A Western blotting was used to detect the H3K4me1 level in ESCC cell lines following SETD7 knockdown or overexpression. B The pie chart shows the distribution patterns of H3K4me1 peaks across genomic functional regions. C The number of genes associated with differentially enriched H3K4me1 peaks (|FC| > 1.5, p < 0.05). D, E The line plot and heatmap showing H3K4me1 signal enrichment around genomic TSS. F, G GO analysis and KEGG analysis for differential genes associated with differential H3K4me1 enrichment identified by CUT&Tag-seq. H Volcano plot showing RNA-seq-derived DEGs between SETD7-knockdown/overexpression and control groups (|FC| > 1.5, p < 0.05). I The GSEA analysis was used for RNA-seq data. The gene set was obtained from FerrDb.