Fig. 5: Liver-specific knockdown of NRF1 reversed the protective effect of DHA against MASH.
From: NRF1 is upregulated by docosahexaenoic acid to ameliorate MASH through the inhibition of ER stress
A Gross observation of livers from WT and shNRF1 mice fed an SCD, pure HFD or HFD supplemented with 4% DHA for 20 weeks. Scale bar, 10 mm. B H&E staining of liver tissues from the abovementioned mice. C Body weight, liver weight and white fat weight of the abovementioned mice (shNC, SCD, n = 9; shNC, HFD, n = 9; shNC, HFD + DHA, n = 5; shNRF1, SCD, n = 9; shNRF1, SCD, n = 10; shNRF1, HFD + DHA, n = 9). D The gene expression of inflammatory factors (Tnfα, Il1β and Il10) in livers of the abovementioned mice (shNC, HFD + DHA, n = 5; n = 8 for other groups). E Western blot analysis of NRF1 and LMP2 expression in livers of the abovementioned mice (n = 4 for each group). F The gene expression of proteasome subunits (Psmb4, Psmb7, Psmc1, and Psmc4) in livers of the abovementioned mice (shNC, HFD + DHA, n = 5; n = 6 for other groups). Two-way ANOVA was used for the statistical analyses in (C, D, and F).
