Fig. 7: NRF1 alleviated oxidative stress in MASH by inhibiting ER stress.
From: NRF1 is upregulated by docosahexaenoic acid to ameliorate MASH through the inhibition of ER stress
A Representative image of DCFH-DA staining of intracellular ROS in AML12 cells treated with FFAs together with DHA and MG132. Scale bar, 200 μm. B Representative images of DCFH-DA staining of intracellular ROS in WT and NRF1-deficient AML12 cells treated with FFAs together with DHA. Scale bar, 200 μm. C Representative images of DCFH-DA staining of intracellular ROS in AML12 cells transfected with NRF1-Flag and subjected to FFAs treatment. Scale bar, 200 μm. D Detection of 8-OH-dG levels in liver tissues from WT and NRF1-overexpressing mice fed the SCD or HFD for 20 weeks. E, F Detection of 8-OH-dG levels in AML12 cells with altered NRF1 expression upon stimulation with FFAs together with tunicamycin or MG132. G Representative images of DCFH-DA staining of intracellular ROS in AML12 cells transfected with NRF1-Flag and stimulated with FFAs together with tunicamycin or MG132. Scale bar, 200 μm. The data were plotted as the means ± SEMs. Two-way ANOVA in (D) and one-way ANOVA in (E, F) were used for the statistical analyses. ns: not significant, * P < 0.05, and *** P < 0.001.
