Fig. 3: TRIM25 interacts with BRD7. | Cell Death & Disease

Fig. 3: TRIM25 interacts with BRD7.

From: TRIM25 degrades BRD7 protein stability through the ubiquitin proteasome pathway to promote breast cancer progression and paclitaxel resistance by activating YB1/Bcl-2 transcription axis

Fig. 3: TRIM25 interacts with BRD7.

A The endogenous interaction between TRIM25 and BRD7 in MDA-MB-231 and MCF7 cells was determined by co-IP assay. B Immunofluorescence assays was performed of TRIM25 (green) and BRD7 (red) colocalization in MDA-MB-231 and MCF7 cells. Scale bar, 10 μm. ImageJ was used to quantitatively analyze the co-localization of TRIM25 and BRD7 in breast cancer cells. C Schematic of the BRD7 protein full length and deletion mutants. D Schematic of the TRIM25 protein, along with the TRIM25 deletion mutants. E Co-IP was performed using an anti-Flag antibody to investigate the interaction between BRD7 and TRIM25 in MCF7 cells co-transfected with HA-BRD7 plasmid or HA-BRD7 truncation mutant plasmids together with Flag-TRIM25 plasmids. F Co-IP analysis of the interaction between TRIM25 and BRD7 in MCF7 cells co-transfected with Flag-TRIM25 deletion mutants together with HA-BRD7 plasmids.

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