Fig. 3: RRN3 interacts with autophagy-related mRNAs and affects autophagy. | Cell Death & Disease

Fig. 3: RRN3 interacts with autophagy-related mRNAs and affects autophagy.

From: Nutrient stress diverts RRN3 from rRNA transcription to alternative polyadenylation of autophagy mRNAs in ovarian cancer

Fig. 3: RRN3 interacts with autophagy-related mRNAs and affects autophagy.

A The schematic diagram of PAR-CLIP method for the identification of RRN3-bound RNAs. B The statistics of RRN3-bound RNA species and the binding locations. C Metagene analysis of RRN3-binding sites along the different regions of mRNAs. D Bar graph showing the distribution of the RRN3-binding sites in different genomic structures. E Gene ontology- and KEGG pathway-enrichment analyses of RRN3-bound mRNAs. F Examples of RRN3-binding sites on autophagy-related mRNAs identified by PAR-CLIP. G The verification of the RRN3 binding on autophagy-related mRNAs by RT-PCR analyses of PAR-CLIP samples. H, I Analyses of autophagy levels of SK-OV-3 cells stably transfected with shRRN3 or control shLacZ and pre-treated with 10 μM CQ for 4 h by western blot (H) or IF (I). The autophagy level was quantified by counting the LC3 puncta numbers in each cell. Data are shown as means ± SEM (***p < 0.001 by unpaired Student’s t-test).

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