Fig. 1: SnRNA-seq identified a cluster of injured podocytes.

A Establishment process of ADR mouse model. Adriamycin was administered via the tail vein and both sides of kidney were harvested for measurements or snRNA-seq. B Representative PAS staining images of renal sections (n = 6). C Urinary albumin to creatinine ratio (ACR) at 0w, 3w and 6w after Adriamycin administration. Data are presented as mean ± SEM and statistical significance was assessed by one-way ANOVA followed by Tukey post-hoc test. ****p < 0.0001 vs 0w. D Umap plot showing 44,525 cells profiled from 6 mouse kidneys of healthy and diseased conditions (n = 3). E Dot plot showing expression of specific markers of each cell cluster. F Absolute proportion of each cell cluster in CON and ADR kidneys revealed by snRNA-seq (n = 3). G Cell proportions in CON and ADR kidneys quantified by bulk RNA-seq deconvolution (n = 3). H The number of DEGs of each cell type between CON and ADR group. I UMAP projection of all podocytes demonstrating original 2 subclusters. J UMAP projection of all podocytes grouped by CON and ADR. K Feature plots showing expression of specific podocyte markers. L UMAP projection of 2 subclusters annotated as Healthy-podo and Injured-podo. M Stacked bar chart showing subcluster cell proportion changes of podocytes in different groups. N Violin plot showing expression of specific podocyte markers in Healthy-podo and Injured-podo. PST proximal straight tubule, PCT proximal convoluted tubule, DTL descending limb of loop of Henle, TAL thick ascending limb of loop of Henle, MD macula densa, DCT distal convoluted tubule, CNT connecting tubule, CD-PC principal cell of collecting, CD-ICA type A intercalated cell of collecting duct, CD-ICB type B intercalated cell of collecting duct, Uro urothelium, Fib fibroblast, MC mesangial cell, Endo endothelial cell, Podo podocyte, Myeloid myeloid cell, Lymphoid lymphoid cell.