Fig. 5: Elovl7 knockdown mitigated the elevation of LC-PUFA-PLs in injured podocytes and reduced podocyte ferroptosis.

A Phospholipid abundance in ADR-treated cells versus CON cells along with the elongation of acyl chain length and the ascending of unsaturation of PL tails. B Heatmap showing relative contents of PE and PC with tails of different length and saturation in wide-type or si-Elovl7 cells with or without ADR treatment. C Relative contents of representative PLs with LC-PUFA tails in wide-type or si-Elovl7 cells with or without ADR treatment (n = 3). Data are presented as mean ± SEM and statistical significance was assessed by one-way ANOVA followed by Tukey post-hoc test. *p < 0.05, **p < 0.01, ***p < 0.001 vs WT group; ^##p < 0.01, ^###p < 0.001 vs “si-NC + ADR” group. D Cell survival rate of cells treated with si-NC or si-Elovl7 after ADR stimulation (n = 5). E GSH/GSSG were detected in cells treated with si-NC or si-Elovl7 after ADR stimulation (n = 3). F Content of MDA in cells treated with si-NC or si-Elovl7 after ADR stimulation (n = 4). Data are presented as mean ± SEM and statistical significance was assessed by one-way ANOVA followed by Tukey post-hoc test. ***p < 0.001, ****p < 0.0001 vs “si-NC” group; ^#p < 0.05, ^##p < 0.01, ^####p < 0.0001 vs “si-NC + ADR” group. G Lipid peroxide in cells treated with si-NC or si-Elovl7 after ADR stimulation stained by Liperfluo (n = 3). H Quantification of Liperfluo intensity in podocytes treated in different condition. Data are presented as mean ± SEM and statistical significance was assessed by one-way ANOVA followed by Tukey post-hoc test. ****p < 0.0001 vs “si-NC” group; ^####p < 0.0001 vs “si-NC + ADR” group. I Immunoblots of Acsl4, Lpcat3 and Elovl7 in cells treated with si-NC or si-Elovl7 after ADR stimulation (n = 3). J Quantification of Acsl4, Lpcat3 and Elovl7 blots normalized to β-actin in si-NC or si-Elovl7 cells after ADR treatment (n = 3). Data are presented as mean ± SEM and statistical significance was assessed by one-way ANOVA followed by Tukey post-hoc test. *p < 0.05 vs si-NC group; ns, not significant.