Table 2 Methods for manipulating the ACBP/DBI system in mice.
Purpose | Category | Method | Reference |
|---|---|---|---|
Activation | Genetic | Transgenic overexpression of Dbi in liver | [24] |
Chemical-genetic system: Expression of a biotin-activatable transgene encoding secreted ACBP/DBI protein in hepatocytes | [45] | ||
Pharmacological | Intravenous injections of recombinant ACBP/DBI protein | [24] | |
Subcutaneous osmotic pumps releasing ACBP/DBI or ACBP/DBI-derived neuropeptides (TTN or ODN) | [45] | ||
Intraperitoneal injection of diazepam for activation of the GABA A receptor | [56] | ||
Inhibition | Genetic | Tamoxifen-inducible, body-wide knockout of Dbi | [24] |
Conditional knockout of Dbi in hepatocytes | |||
Constitutive mutation F77I in Gabrg2, blocking interaction with ACBP/DBI | |||
Immunological | Autoimmunization with ACBP/DBI-KLH conjugate | ||
Monoclonal antibodies neutralizing ACBP/DBI injected i.v. or i.p. |
