Fig. 1: Disrupted 14-3-3 protein function promotes BAD translocation. | Cell Death & Disease

Fig. 1: Disrupted 14-3-3 protein function promotes BAD translocation.

From: A high-throughput screening approach to discover potential colorectal cancer chemotherapeutics: repurposing drugs to identify novel disruptors of 14-3-3 proteins

Fig. 1

A NIH-3T3 cells were made to express mCitrine-conjugated BAD (BAD) or BAD mutants harboring S112A and S136A double mutations (BAD-AA). BAD-expressing cells were treated with either R18 (10 µM) or FTY720 (2 µM) for 24 h. The mitochondrial membrane potential sensor TMRE (100 nM) was added to the medium 1 h prior to live-cell imaging. Representative images were selected from three independent experiments, with at least three images were captured per dish in each experiment (Scale bar = 10 µm). B The co-localization of BAD and mitochondria in 1 A was assessed by calculating Pearson’s correlation coefficient (R). Each data point corresponds to a single cell. Statistical significance was determined using one-way ANOVA with Tukey’s post hoc test. *P < 0.05; ***P < 0.001.

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