Table 1 Primers and restriction enzymes for the construction of BRET sensor.

From: A high-throughput screening approach to discover potential colorectal cancer chemotherapeutics: repurposing drugs to identify novel disruptors of 14-3-3 proteins

Gene

Primer

5’-14-3-3ζ

ATGGATAAAAATGAGC

3’-14-3-3ζ

ATTTTCCCCTCCTTCTCCTG

5’-BAD

ATGGGAACCCCAAAGCAG

3’-BAD

TGGATCCTGGGAGGGGGTG

3’-BAD-136F

CGCTGCCCAGAGATTGGG

5’-112-136F

ATGGAGACTCGGAGTCGC

5’-Rluc8

ATGGCTTCCAAGGTGTACGAC

3’-Rluc8

CTGCTCGTTCTTCAGCACGC

5’mCitrine

ATGGTGAGCAAGGGCGAG

3’-mCitrine

CTTGTACAGCTCGTCC

5’-mTurquoise

ATGGTGAGCAAGGGCG

3’-mTurquoise

CTTGTACAGCTCGTCCATGCC

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