Fig. 5: CARMN knockdown transcriptionally downregulates autophagy via the AKT/ATG7 pathway. | Cell Death & Disease

Fig. 5: CARMN knockdown transcriptionally downregulates autophagy via the AKT/ATG7 pathway.

From: CARMN loss promotes VSMC-derived foam cell formation and atherosclerosis through transcriptional downregulation of autophagy

Fig. 5: CARMN knockdown transcriptionally downregulates autophagy via the AKT/ATG7 pathway.The alternative text for this image may have been generated using AI.

A The Venn plot of the differentially expressed ATGs. B The GO enrichment analysis was performed to identify the biological process (BP), cellular component (CC), and molecular function (MF) of the differentially expressed ATGs. C Western blotting analysis of total and phosphorylated forms of AKT and mTOR in VSMCs treated with ASO-NC or ASO-CARMN. D Quantification of total and phosphorylated levels of AKT and mTOR (n = 3). E Western blotting analysis of total and phosphorylated forms of AKT in VSMCs incubated with MK2206 (5 µM) for 6 h before treatment with ASO-NC or ASO-CARMN. F Quantification of total and phosphorylated levels of AKT (n = 3). G Western blotting analysis of LC3 and ATG7 in VSMCs incubated with MK2206 (5 µM) for 6 h before treatment with ASO-NC or ASO-CARMN. H Quantification of LC3 II/LC3 I and ATG7 protein expression (n = 3). Data are presented as mean ± SD. Statistical analysis was performed using one-way ANOVA for multiple-group comparisons. ns, P > 0.05; ***, P < 0.001; ****, P < 0.0001.

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