Fig. 4: CNOT7 knockdown impairs NHEJ mediated DSB repair. | Cell Death & Disease

Fig. 4: CNOT7 knockdown impairs NHEJ mediated DSB repair.

From: CNOT7 facilitates radiation resistance in colorectal cancer through TRIM21/XRCC6-mediated non-homologous end joining repair

Fig. 4: CNOT7 knockdown impairs NHEJ mediated DSB repair.

A Western blotting of HR and NHEJ related protein expression at different time points after radiation treatment in HCT116 and CNOT7 knockdown HCT116 cell lines. B Western blotting of homologous recombination (HR) and non-homologous end joining (NHEJ) related protein expression at different time points after radiation treatment in SW480 and CNOT7 overexpression SW480 cell lines. C RT-qPCR analysis of the expression levels of homologous recombination (HR) and non-homologous end joining (NHEJ) related genes in HCT116 and CNOT7 knockdown HCT116 cell lines. D RT-qPCR analysis of the expression levels of homologous recombination (HR) and non-homologous end joining (NHEJ) related genes in SW480 and CNOT7 overexpression SW480 cell lines. E, F NHEJ and HR repair efficiency were assessed in HCT116 cells transfected with DR-GFP and EJ5-GFP reporter plasmids. G Representative immunohistochemistry (IHC) images showing the expression of CNOT7, XRCC6, and 53BP1 in HCT116 and CNOT7 knockdown HCT116 cell-derived subcutaneous tumors with or without radiotherapy. Scale bar: 60 μm.

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