Fig. 3: JQ1 increased TXNIP expression in the presence of glucose and inhibited histone H3K9 trimethylation.
From: BRD4 inhibition suppresses histone H4 UFMylation to increase ferroptosis sensitivity through TXNIP
A, B The protein (A) and mRNA (B) levels of TXNIP after JQ1 treatment with or without glucose. C The enrichment of BRD4 and RNA Pol II at TXNIP promoter in cells cultured with or without glucose. D The expression of TXNIP after JQ1 treatment with or without glutamine. E The distribution of MLX in cells treated with JQ1. Nuclear and cytosolic fraction were prepared and probed with MLX antibody. F The enrichment of MLX and RNA Pol II at TXNIP promoter in cells treated with or without JQ1. G JQ1 decreased H3K9Me3 and H3K27Me3 levels in HepG2 cells. Whole cell extracts were prepared and probed with indicated antibodies. H The levels of BRD4, H3K9Me3, and H3K27Me3 at TXNIP promoter in cells treated with or without JQ1. I The protein levels of TXNIP and H3K9Me3 in cells treated by JQ1, ML324, and a combination of JQ1 and ML324. J The mRNA levels of TXNIP in cells treated by JQ1, ML324, and a combination of JQ1 and ML324. ***p value < 0.005, **p value < 0.01, *p value < 0.05 (two-tailed unpaired Student t test in B, C, F, H, and J); ns not significant.
