Fig. 7: Impact of DNMT1 on RASSF1A Methylation.
From: DNMT1 modulation of RASSF1A methylation enhances breast cancer brain metastasis
A, B RT-PCR and Western blot to assess the effect of DNMT1 overexpression or knockdown on RASSF1A mRNA and protein levels in 4T1 cells. C, D RT-PCR and Western blot to examine the impact of 5-Aza-CdR on DNMT1 and RASSF1A mRNA and protein levels in 4T1 cells. E MSP to detect the Methylation status of RASSF1A in each group (M Methylation-specific primers for MSP, U MSP using non-Methylation-specific primers; bands at 52 bp and 44 bp indicate the presence of unmethylated and methylated sequences). F ChIP experiment to assess DNMT1 enrichment on the RASSF1A promoter. All cell experiments were repeated thrice; ns denotes no significant difference between groups, ** indicates statistical significance at P < 0.01, and *** at P < 0.001.
