Fig. 2: MSCs inhibits HG-induced mitochondrial fragmentation in a Drp1-dependent manner. | Cell Death & Disease

Fig. 2: MSCs inhibits HG-induced mitochondrial fragmentation in a Drp1-dependent manner.

From: MSCs ameliorates hyperglycemia-induced endothelial injury through modulation of mitochondrial dynamics

Fig. 2

A–E Mitochondrial dynamics-related proteins, including Drp1, Fis1, MFN2, and OPA1 in the HUVECs were analyzed by Western blots (n = 5 independent experiments). F–J Representative Western blots and densitometric analysis of Drp1, Fis1, MFN2 and OPA1 protein expression in mouse aortas (n = 6 mice/group). K Lysates immunoprecipitated with anti-Drp1 antibody were used to measure Drp1 GTPase enzyme activity (n = 3 independent experiments). L Cytoplasmic and mitochondrial fractions from HUVECs were analyzed by Western blots, the fractions were probed for Drp1. M HUVECs were transfected with DN-Drp1, empty vector or cocultured with MSCs, cells were then incubated with HG for 48 h, micrographs of mitochondrial morphology were visualized by anti-COXIV antibody staining (n = 60–100 cells/group). Data are shown as means ± SD. ns indicates not significant. *P < 0.05. Data were analyzed using one-way ANOVA with Tukey’s post hoc test.

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