Fig. 3: MSC-derived STC1 counteracts HG-induced mitochondrial fission and mitochondrial compromise in a Drp1-dependent manner. | Cell Death & Disease

Fig. 3: MSC-derived STC1 counteracts HG-induced mitochondrial fission and mitochondrial compromise in a Drp1-dependent manner.

From: MSCs ameliorates hyperglycemia-induced endothelial injury through modulation of mitochondrial dynamics

Fig. 3: MSC-derived STC1 counteracts HG-induced mitochondrial fission and mitochondrial compromise in a Drp1-dependent manner.The alternative text for this image may have been generated using AI.

A HUVECs were transfected with a scramble siRNA or siRNAs directed against the candidate secretory molecules, lysates immunoprecipitated with anti-Drp1 antibody were used to measure Drp1 GTPase activity (n = 5 independent experiments). Data are shown as means ± SD. (*P < 0.05 vs. Conn, &P < 0.05 vs. HG, #P < 0.05 vs. HG+MSCs+scramble). B STC1 levels in culture media of HUVECs from different groups were detected by ELISA (n = 3 independent experiments). Data are shown as means ± SD. ns indicates not significant. *P < 0.05. C STC1 levels in culture media of HUVECs, MSCs, and HSFs in the presence or absence of HG incubation were quantified by ELISA (n = 3 independent experiments). Data are shown as means ± SD. *P < 0.05. D Representative Western blots and densitometry analysis of mitochondrial Drp1 in HUVECs upon HG insults when cocultured with MSCs transfected with scramble siRNA or with STC1 siRNA (n = 5 independent experiments). Data are shown as means ± SD. *P < 0.05. E Micrographs of mitochondrial morphology were visualized by anti-COXIV antibody staining in HUVECs cocultured with MSCs transfected with scramble siRNA or with STC1 siRNA (n = 60–100 cells/group). F Representative Western blots and densitometry analysis of mitochondrial Drp1 in HUVECs treated with rSTC1 (50 ng/mL), anti-rSTC1 antibody or control IgG (n = 5 independent experiments). Data are shown as means ± SD. *P < 0.05. G Micrographs of mitochondrial morphology were visualized by anti-COXIV antibody staining in HUVECs treated with rSTC1, anti-rSTC1 antibody or control IgG (n = 60–100 cells/group). H–J Basal and maximal mitochondrial oxygen consumption rates (OCRs) in HUVECs were determined by Seahorse XF96 analyses (n = 3 technical replicates). K ATP levels were quantified in HUVECs (n = 3 independent experiments). Data are shown as means ± SD. *P < 0.05. Student’s t test (unpaired) for the comparisons of two groups, and one-way ANOVA with Tukey’s post hoc test if more than two groups were compared.

Back to article page