Fig. 4: STC1 regulates mitochondrial dynamics remodeling through ERK1/2-Drp1 axis.
A, B HUVECs were treated with rSTC1 for indicated times, and Drp1-Ser637 and Drp1-Ser616 protein levels were detected by Western blot (n = 3 independent experiments). Data are shown as means ± SD. *P < 0.05. C, D HUVECs were treated with rSTC1 for indicated times, and p-ERK1/2 protein levels were detected by Western blot (n = 3 independent experiments). Data are shown as means ± SD. *P < 0.05. E, F HUVECs were incubated with HG for 24 h or 48 h, and lysates were Western blotted for indicated proteins (n = 3 independent experiments). Data are shown as means ± SD. (*P < 0.05 vs. Conn, &P < 0.05 vs. HG24h) G–I HUVECs were incubated with HG in the presence or absence of PD98059 (10 μM) for 48 h, and lysates were Western blotted for indicated proteins (n = 3 independent experiments). Data are shown as means ± SD. *P < 0.05. J, K Representative Western blots and bar graphs of Drp1 of mitochondrial fractions obtained from HUVECs in each group (n = 5 independent experiments). Data are shown as means ± SD. *P < 0.05. L–N MSCs transfected with scrambled or STC1-specific siRNA were co-cultured with HUVECs in medium containing HG, representative Western blots and densitometry analysis of p-Drp1 and p-ERK1/2 levels in each group (n = 3 independent experiments). Data are shown as means ± SD. *P < 0.05. O–Q, HUVECs were exposed to HG with or without rSTC1, control IgG or anti-STC1 antibodies were added to the HUVECs, and lysates were Western blotted for indicated proteins (n = 3 independent experiments). R, S Representative Western blots and bar graphs of Drp1 in mitochondrial fractions obtained from HUVECs in each group (n = 5 independent experiments). Data are shown as means ± SD. *P < 0.05. Data were analyzed using one-way ANOVA with Tukey’s post hoc test.
