Fig. 2: TMEM166 negatively regulates UPR. | Cell Death & Disease

Fig. 2: TMEM166 negatively regulates UPR.

From: TMEM166 negatively regulates unfolded protein response to affect hepatocellular carcinoma cell growth and sorafenib resistance

Fig. 2

A Immunoblotting of the indicated UPR proteins in whole cell lysates or nuclear lysates extracted from Control and Cas9-TMEM166/Huh7 cells. Phosphorylation of PERK is recognized as a band shift to a higher molecular weight. B Quantification of XBP1s, ATF4 and nuclear cleaved ATF6 relative to GAPDH or nuclear Histone 3 in Control and Cas9-TMEM166/Huh7 cells. Average value in Control cells was normalized as 1. C Control and Cas9-TMEM166/Huh7 cells treated with or without Tm (0.5 μg/mL, 6 h, 1 μg/mL, 6 h) or Tg (0.2 μM, 6 h). The relative mRNA levels of the indicated genes were detected by qRT-PCR. D Immunoblotting of the indicated UPR proteins in Control and Cas9-TMEM166/Huh7 cells stably expressing vector or TMEM166. E BEL-7402 cells were transfected with indicated plasmids for 48 h, then the indicated UPR proteins in whole cell lysates or nuclear lysates were detected by immunoblotting. F Quantification of XBP1s, ATF4 and nuclear ATF6 relative to GAPDH or nuclear Histone 3 in indicated cells. Average value in BEL-7402 cells over-expressing GFP-vector was normalized as 1. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Data (mean ± SD) are representative of at least three independent experiments.

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