Fig. 3: DazlE8KO male mice exhibit meiotic defects.
From: Loss of full-length DAZL isoform disrupts PABPC1-dependent translational regulation and meiosis
A Immunofluorescent double labeling of γH2AX (green) and SYCP3 (red) in mouse leptotene, zygotene, pachytene, diplotene spermatocytes during metaphase I. Representative images of DSB repair defects and impaired XY body formation in DazlE8KO spermatocytes are displayed on the right panel. B Quantitative analysis of spermatocyte populations and defects in DSB repair and XY body formation in wildtype and DazlE8KO males. Spermatocyte counts were 1151 (wildtype) and 1285 (DazlE8KO). Data presented are mean ± SD, P values are annotated in the histograms. C H&E staining in testis sections of P35 wildtype and DazlE8KO mice. Red arrowheads in red dashed boxes indicate misaligned chromosomes in metaphase I spermatocytes. The histogram represents the rate of chromosome misalignment. Data presented are mean ± SD. Scale bar, 20 μm.
