Fig. 4: The absence of DAZL_FL protein disrupts the binding of DAZL to the translation machinery, but does not affect its enrichment of target genes.

A Integrative genomics viewer (IGV) genome tracks showing DAZL binding peak distributions on the 3′ UTR of meiosis-associated genes. B RNA immunoprecipitation (RIP) was performed in WT and Dazl^E8KO testes, and GAPDH was used to quantify. C RIP-qPCR assays showing the enrichment levels of DAZL′s targets in the testes of both WT and Dazl^E8KO mice, with the non-target genes Crem and Acrv1 serving as a negative control. Data are presented as mean ± SD, with P values indicated. D Sliver staining of DAZL immunoprecipitation (IP) in the testes of wildtype (WT) and Dazl^E8KO mice. IgG IP used as a negative control. E Venn network for DAZL interactomes in WT and Dazl^E8KO testes. 30 proteins are shared by the two groups. The wildtype and Dazl^E8KO groups possess 289 and 72 specific proteins, respectively. F Gene ontology (GO) enrichment analysis of 289 unique genes in WT group. G Co-IP assays showing the interactions between DAZL and EIF4G3 or PABPC1 in WT and Dazl^E8KO testes. Non-immune IgG was used as a negative control. H Western blot showing Co-IP of HA-tagged PABPC1 with either over-expressed MYC-tagged DAZL_FL or DAZL_Sh in HEK293T cells. IgG was used as a negative control.