Fig. 1: Expression of DJ-1 was reduced after CVB3 infection.

A Quantitative qRT-PCR analysis of DJ-1 mRNA levels in the control and CVB3-infected H9C2 cells. B Western blot analysis of DJ-1 protein expression in H9C2 cells. Ctrl negative control (MEM), CVB3 CVB3 infected groups. C Quantitative qRT-PCR analysis of DJ-1 mRNA levels in the heart of Control and VMC mice (n = 3). D Western blot analysis of DJ-1 protein expression in the heart of Control and VMC mice (n = 3). E Immunofluorescence staining of DJ-1 in the heart of control and VMC mice. Nuclei were counterstained with DAPI (blue); red staining indicates DJ-1 exists (scale bar:50 μm, n = 6). (*P < 0.05; **P < 0.01).