Fig. 6: DJ-1 deficiency downregulates Dusp1 gene expression. | Cell Death & Disease

Fig. 6: DJ-1 deficiency downregulates Dusp1 gene expression.

From: Protein deglycase DJ-1 deficiency aggravates acute viral myocarditis by promoting apoptosis via reducing Dusp1 expression

Fig. 6

A RNA transcriptome sequencing of the heart from DJ-1−/− and WT mice. The fold change of all genes with a significant difference was presented in the volcano plot. B KEGG analysis of downregulated genes in DJ-1−/− mice compared with the WT type. C H9C2 cells were transfected with siRNA targeting DJ-1 (si-DJ-1) or a control scrambled sequence (si-NC). The mRNA levels of DJ-1 and Dusp1 were determined by RT-qPCR at the indicated time. D Quantitative RT-qPCR analysis of Dusp1 mRNA level in the heart of DJ-1 deletion mice and wild-type mice. E–H Depressed protein level of Dusp1 and increased activation of the P38MAPK pathway in the DJ-1 deficiency groups compared with the control (the data are represented as the mean ± SEM. One-way ANOVA by post-test (Tukey) analysis was used. n = 3. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001).

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