Fig. 5: YTHDF3/m6A/STAT3 suppresses antitumor immunity and creates an ITME.
A PCR analysis validated the knockout efficiency of the Ythdf3 gene in Ythdf3−/− mice (n = 2). B Viral titers in tumor tissues collected on day 10 postinfection were measured via qPCR in Ythdf3+/+ and Ythdf3−/− mice, and HPV DNA levels were reduced in the latter group (n = 3). C The production of IFN-α in the serum of wild-type (WT) mice and Ythdf3−/−mice was measured (n = 3). D IHC staining was used to visualize YTHDF3 protein expression in the tumor tissues of wild-type (WT) mice and Ythdf3−/− mice (n = 3). E IHC analysis of E6 protein expression in tumor tissues from wild-type (WT) mice and Ythdf3−/− mice (n = 3). F IHC analysis of STAT3 and IRF7 protein expression in the tumor tissues of Ythdf3−/− mice and control mice. G The percentage of CD8+ T cells in the tumor tissues of Ythdf3−/− mice and wild-type mice was determined (n = 3). H The percentages of Tregs in Ythdf3−/− and wild-type mice were evaluated (n = 3). I The frequency of M2 macrophages was detected in the tumor tissues of Ythdf3−/−mice and wild-type mice (n = 3). J The percentage of MDSCs in the tumor tissues of Ythdf3−/− mice and wild-type mice (n = 3).
