Fig. 1: Baseline differences in lipid peroxidation sensitivity and lipid composition in OVCAR-3 and Caov-3 cells. | Cell Death & Disease

Fig. 1: Baseline differences in lipid peroxidation sensitivity and lipid composition in OVCAR-3 and Caov-3 cells.

From: Photodynamic therapy simultaneously induces ferroptosis- and apoptosis-like lipid signatures in ovarian cancer cells

Fig. 1: Baseline differences in lipid peroxidation sensitivity and lipid composition in OVCAR-3 and Caov-3 cells.

A Metastatic and relatively chemoresistant OVCAR-3 cells are more sensitive to Cum OOH-induced lipid peroxidation than primary and chemosensitive Caov-3 cells. B Dose-response curves showing differential sensitivity to Cum OOH between OVCAR-3 and Caov-3 cells. C Oxidized C11 BODIPY fluorescence in OVCAR-3 and Caov-3 cells treated with 50 μM Cum OOH for 1.5 h. Blue – Hoechst fluorescence, green – oxidized C11 BODIPY fluorescence. D Relative abundances of select lipid classes in OVCAR-3 and Caov-3 cells. PC phosphatidylcholines, SM sphingomyelins, ePC ether-linked phosphatidylcholines, ePE ether-linked phosphatidylethanolamines, SPH sphingosines, PE phosphatidylethanolamines. Volcano plot (E) and a heatmap (F) depicting differentially expressed PCs between OVCAR-3 and Caov-3 cells. Volcano plot (G) and a heatmap (H) depicting differentially expressed PEs between OVCAR-3 and Caov-3 cells. Arrows indicate an increase in unsaturation index calculated as the total carbon chain length divided by the total number of double bonds. Peak areas of PC(40:10) (I) and PE(P-34:6) (J) as representative polyunsaturated phospholipids. Statistics: B Each point represents the mean of three biological replicates, each conducted in duplicate. Gray area denotes the null zone with non-overlapping 95% confidence intervals. E, G Each data point represents the mean of four biological replicates. Differentially expressed lipids are defined as those with absolute log2 fold change > 1 and p-value < 0.05 (two-tailed unpaired t-test). F, H Heatmaps represent z scores of lipid species with absolute log2 fold change > 1 and p-value < 0.05 (two-tailed unpaired t-test), four biological replicates. I, J Each dot represents a biological replicate; error bars represent the range. Statistical significance was determined using two-tailed unpaired t-test. *p ≤ 0.05, ***p ≤ 0.001. Heatmaps and the null zone dose-response plot were created in R (version 4.4.1 2024-06-14), volcanos and spread plots were created with Graphmatik (version 0.3.2, 2025).

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