Fig. 3: TIGIT expression and phenotypic characteristics of BCMA-CAR-T cells following antigen activation. | Cell Death & Disease

Fig. 3: TIGIT expression and phenotypic characteristics of BCMA-CAR-T cells following antigen activation.

From: Preclinical evaluation of TIGIT as a target to enhance efficacy and mitigate T cell exhaustion in multiple myeloma following BCMA-CAR-T therapy

Fig. 3

A Representative histograms showing TIGIT expression on total CAR-T cells (left), CD4+ CAR-T cells (middle), and CD8+ CAR-T cells (right) at different antigen concentrations. B TIGIT expression levels on CAR-T cells under varying antigen concentrations. C TIGIT expression levels on CAR-T cells after co-culture with antigen over time. D Flow cytometric gating strategy for analyzing CAR-T cells phenotypes: naïve and stem cell-like memory (TN/TSCM; (CD45RO– CD62L + ), central memory (TCM; CD45RO+ CD62L+), effector memory (TEM; CD45RO+ CD62L–), and terminally differentiated effector (TTE; CD45RO– CD62L–). E Representative density plots showing CAR-T cell phenotypes after co-culture with target cells at different antigen concentrations.(left: total CAR-T cells; middle: CD4+ CAR-T cells; right: CD8+ CAR-T cells). F Quantification of CAR-T cell phenotypes after co-culture with target cells at varying antigen concentrations (left: total CAR-T cells; middle: CD4 + CAR-T cells; right: CD8 + CAR-T cells). G Temporal changes of CAR-T cell phenotypes after co-culture with MM cells. H Comparison of phenotypes between TIGIT+ and TIGIT– CAR-T cells (Data are presented as mean ± SEM).

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