Fig. 7: RNA-seq analysis of the transcriptional impact of TIGIT knockout in CAR-T cells. | Cell Death & Disease

Fig. 7: RNA-seq analysis of the transcriptional impact of TIGIT knockout in CAR-T cells.

From: Preclinical evaluation of TIGIT as a target to enhance efficacy and mitigate T cell exhaustion in multiple myeloma following BCMA-CAR-T therapy

Fig. 7

A Experimental design of RNA-seq: CAR-T cells were co-cultured with MM cells at a high E:T ratio (2:1) for 1 day (activated CAR-T cells) or at a low E:T ratio (0.1:1) for 7 days (exhausted CAR-T cells). CAR-T cells were isolated by magnetic sorting for RNA-seq. B Heatmap showing clustering of gene expression across groups. EC: activated EC-CAR-T; EC-Ex: exhausted EC-CAR-T; TigitKO: activated TIGIT-knockout CAR-T cells; TigitKO-Ex: exhausted TIGIT-knockout CAR-T cells. C Venn diagram showing DEGs across groups. D GO enrichment analysis of the 116 intersecting DEGs. E KEGG enrichment analysis of the 116 intersecting DEGs. F PPI network of the 116 intersecting DEGs, with the cluster containing TIGIT highlighted in light red (lower left).

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