Fig. 8: Preliminary functional validation of TIGIT downstream molecules. | Cell Death & Disease

Fig. 8: Preliminary functional validation of TIGIT downstream molecules.

From: Preclinical evaluation of TIGIT as a target to enhance efficacy and mitigate T cell exhaustion in multiple myeloma following BCMA-CAR-T therapy

Fig. 8: Preliminary functional validation of TIGIT downstream molecules.

A Circular heatmap of expression differences for genes in the TIGIT-containing cluster. B PPI network of genes within the TIGIT-associated cluster. C Validation of knockdown efficiency for candidate genes by qPCR 48 h after siRNA transfection. D Effect of gene knockdown on CAR-T degranulation assessed by CD107a assay. E Representative plots of CAR-T degranulation after knockdown of different genes. F CAR-T cell counts on days 4 and 7 after siRNA-mediated gene knockdown (Data are presented as mean ± SEM; *P < 0.05).

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