Fig. 1: Increased H3K4me3 levels induced by folate deficiency bind neurodevelopment-related gene promoters in the wnt/β-catenin pathway. | Cell Death & Disease

Fig. 1: Increased H3K4me3 levels induced by folate deficiency bind neurodevelopment-related gene promoters in the wnt/β-catenin pathway.

From: Loss of KDM5A-mediated H3K4me3 demethylation promotes aberrant neural development by Wnt/β-catenin pathway activation

Fig. 1

A Comparison of average H3K4me3 ChIP-seq read densities between control and folate-deficient C57mESCs. B H3K4me3 ChIP-seq enrichment profiles of control and folate-deficient mESCs. C Pathway analysis of H3K4me3 ChIP peaks via PANTHER. The x-axis shows the enrichment factor, the y-axis shows the binomial raw P value, and the size of each circle represents the number of genes in the pathway. D GO enrichment analysis of H3K4me3 ChIP peaks. The x-axis shows the enrichment factor, the y-axis shows the binomial raw P value, and the size of each circle represents the number of genes in the GO term. E H3K4me3 ChIP assays were performed using C57mESCs subjected to folate deficiency for 6 passages. Mouse IgG was used as a control. The enrichment of H3K4me3 in the promoters of the neurodevelopment-related genes Axin2, Bcl9l, Atoh1, Nkx2.2, Sox1 and Isl1 was measured via RT‒qPCR. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. F H3K4me3 ChIP assays were performed using mouse NE4C cells subjected to folate deficiency for 6 passages. Mouse IgG was used as a control. The enrichment of H3K4me3 in the promoters of the neurodevelopment-related genes Axin2, Bcl9l, Atoh1, Nkx2.2, Sox1 and Isl1 was measured via RT‒qPCR. *p < 0.05, **p < 0.01, ***p < 0.001. E, F are presented as the means ± SEMs from three biological replicates.

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