Fig. 6: Wnt targets genes were upregulated in KDM5A-KO zebrafish. | Cell Death & Disease

Fig. 6: Wnt targets genes were upregulated in KDM5A-KO zebrafish.

From: Loss of KDM5A-mediated H3K4me3 demethylation promotes aberrant neural development by Wnt/β-catenin pathway activation

Fig. 6

A Scheme of experiment design upon 24hpf zebrafish with KDM5A-CRISPR/Cas9. B Morphology of 24hpf zebrafish embryos with KDM5A knocking down with CRISPR/Cas9. Scale bar, 0.19 mm. The class I and Class II showed NTDs-like aberrant neurodevelopment. The abnormal ratio of 24hpf zebrafish embryos with KDM5A knocking down were showed in right panel with three replicate experiments. C GO enrichment analysis in biological process, cellular component and molecular function of different genes upon KDM5A knocking down with CRISPR/Cas9. The x-axis shows the Rich Ratio the y-axis shows the binomial pathway, and the size of each circle represents the number of genes. D Heatmap analysis of wnt target genes in 24hpf zebrafish upon KDM5A knockout with CRISPR/cas9. E In situ hybridization for Wnt target genes in the 24hpf zebrafish with KDM5A CRISPR/Cas9 injection. The size of zebrafish used for experiment were shown in the lower right corner of each image. The denominator represents the total number of samples, and the numerator represents the number of samples with positive results respectively.

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